454 Bio has developed new chemistry, hardware, and analysis software that make on-site sequencing more accessible than ever before. We are releasing it to the world so that anyone can get started.
How it works
Our patented Lightning Terminators (LTs) illuminate clusters of bases.
Unlike others, our process does not require fluidics of any kind — 454 sequencing is done in One Pot with mostly off-the-shelf hardware.
LTs are cleaved from bases with ultraviolet light, meaning that TIRF alone is sufficient to both monitor and maintain the reaction.
Applications
454 Bio’s technology enables secure, rapid, and portable DNA sequencing, unlocking countless possibilities including:
Rapid pathogen identification, Personalized at-home health assessments, such as cancer risk screening, Environmental DNA monitoring in the field, and Privacy-first DNA sequencing without third party data handling
Get started
Prepare your samples
For successful DNA sequencing, carefully prepare your samples by following sterile collection methods and precise DNA extraction and purification protocols. Click the ‘Read more’ button below for detailed instructions and best practices.
Build your sequencer
Download hardware CAD models, PCB layouts, the complete bill of materials, and instrument and analysis software.